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Western blotting for AM peptide in AGS cells. Cells were exposed to either the invasive or noninvasive species of E. coli for 1 h, and cell extracts were immunoblotted for AM. Lane 1, nontreated cells; lane 2, cells exposed to E. coli E35990; lane 3, preabsorbed primary antibody (negative control) on nontreated cells; lane 4, cells exposed to E. coli DH5α.
