FIG. 3.

CH1313 was inoculated twice with HEV, first with the Pakistani strain and then, 45 months later, with the Mexican strain (36). Week zero represents a convalescent-phase sample before the second inoculation, and subsequent samples were drawn weekly. Samples were tested for IgM anti-HEV by the class capture method and for IgG anti-HEV by the sandwich assay. Samples were also treated with 0.01 M DTT before being tested for IgM anti-HEV by the class capture method. Samples treated with DTT and samples treated with PBS were analyzed on the same plate. Plus and minus signs at the top are results from the IgM anti-HEV sandwich EIA, as previously published (36).