TABLE 1.
Assay | IgM concn | No. of each sample testeda | OD
|
||
---|---|---|---|---|---|
Mean | SD | % CV | |||
Intraplate precision | Low | 28 | 0.461 | 0.022 | 4.800 |
Intermediate | 28 | 1.418 | 0.062 | 4.371 | |
High | 28 | 2.154 | 0.036 | 1.674 | |
Interplate precision | Low | 12 | 0.469 | 0.029 | 6.263 |
Intermediate | 12 | 1.585 | 0.065 | 4.124 | |
High | 12 | 2.146 | 0.079 | 3.671 |
All samples were from the same pool. For the interplate precision assay, samples were aliquoted, frozen, and tested in separate plates.