Figure 1.

The magnitude of cholestasis is similar in Ctsb^+/+, Ctsb^–/–, and R-3032–treated Ctsb^+/+ mice. Ctsb^+/+, Ctsb^–/–, and R-3032–treated Ctsb^+/+ mice underwent a sham operation (control) or ligation of their common bile duct. Three days after the surgical procedure, the mice were anesthetized, and liver tissue and serum were obtained. (a) Fixed liver specimens from all mice were stained by conventional hematoxylin and eosin. Bile duct proliferation, portal edema, and mild portal infiltrates were present in all BDL mice. However, bile infarcts only occurred in Ctsb^+/+ BDL mice. (b and c) Serum total bilirubin and total bile acids in BDL mice were comparable among the three groups.