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. 2003 Jul 15;112(2):181–188. doi: 10.1172/JCI17778

Figure 2.

Figure 2

M5 Ab inhibited migration of splenic monocytes from arthritic mice. (a) Specificity of M5 Ab against OPN peptides was examined by analysis using BIAcore analysis of M5 Ab. Vertical and horizontal axes indicate surface plasmon resonance intensity (units) and flow time of HBS buffer, respectively. M5 Ab was injected at 200 seconds over the surface of the chip and then washed with HBS buffer. The binding of M5 Ab to GRGDSLAYGLR peptide (green), SLAYGLR peptide (red), and GRGDS peptide (blue) is shown. (b) Inhibition by M5 Ab of cell migration toward thrombin-cleaved OPN. M5 Ab, antipolyclonal OPN Abs, anti-β3 integrin Ab, anti-CD44 Ab, and isotype-matched control antibodies were added at the indicated concentrations. Anti–N-terminal OPN was added at a 1:100 dilution to wells. The results are expressed as mean numbers ± SEM of migrated cells. *P < 0.05 and **P < 0.01. Resp. diff., response difference.