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The fluorescence quenching ratio of 2-OF is a sensitive function of ionic strength. The concentration of 2-OF is 1.0 × 10—6 M (in monomer repeat units) and that of the 5-nm gold nanoparticles is 3.4 × 10—10 M. The buffer concentration is in units of c0: 1 c0 of SSC buffer contains 0.015 M sodium citrate and 0.15 M NaCl, at pH 7.0.
