Fig. 1.

Arrangement of injected DNA and PCR screen of candidate transgenic mice. (A) Sequences in 5′eyfp and 3′eyfp are represented by two large arrows with emphasis on the coding sequences (hatched), deleted regions (black), and sequences unique to each cassette (gray). Promoter, intron, and polyadenylation signal (pA) sequences are indicated. Expression is driven by the chicken β-actin promoter and cytomegalovirus enhancer. (B) PCR analysis of candidate mice with primers that anneal to EYFP coding sequence as shown in A. Template DNA was from mouse cells expressing EGFP (lane 1), wild-type mouse cells (lanes 2 and 3), and a subset of candidate mice (lanes 4–10). M, size standards.