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. 2003 May 15;100(11):6325–6330. doi: 10.1073/pnas.1232231100

Fig. 5.

Fig. 5.

Analysis of the recombination mechanism in a recombinant clone. Diagrams depict coding sequences (cds) only. Common sequences are in white, essential terminal coding sequences are in gray, and deleted sequences are in black. (A) Primers that amplify coding sequences yield the indicated products (Upper). PCRs of template DNA from plasmids carrying EYFP (lane 2), 5′eyfp (lane 3), and 3′eyfp (lane 4) are shown. Lane 1 shows size markers, and lane 5 shows a mixture of PCR products from lanes 2–4. (B) PCR results from yellow fluorescent clone Y1 (lane 1) and a mixture of control PCR products (lane 2). (C) Schematic diagram of gene conversion. B, BamHI. (D) BamHI-digested DNA from the nonfluorescent parent population (lane 1) and fluorescent clone Y1 (lane 2), probed with EYFP coding sequences. Note that the degraded downstream 3′eyfp cassette (Fig. 3D) cannot participate in events that reconstitute EYFP, cannot be amplified by using the primers indicated in A, does not yield a detectable signal by Southern analysis, and thus is not depicted in C.