Table 2. Analyses of the embryos and the genotypes of the intact embryos at various stages of their development.
| Genotypes
|
|||||
|---|---|---|---|---|---|
| Embryonic stage | Litter size | Resorbed embryos | WT | +/- | -/- |
| E14.5 | 9 | 3 | 2 | 4 | 0 |
| E13.5 | 8 | 3 | 2 | 3 | 0 |
| E12.5 | 9 | 0 | 5 | 4 | 0 |
| E10.5 | 10 | 3 | 3 | 4 | 0 |
| E9.5 | 10 | 2 | 3 | 5 | 0 |
| E8.5 | 9 | 0 | 5 | 4 | 0 |
| 10 | 5 | 3 | 2 | 0 | |
| 3 | 0 | 0 | 3 | 0 | |
| E7.5 | 6 | 0 | 3 | 3 | 0 |
| E6.5* | 10 | 2 without embryos | |||
| 1 resorbed, 1 retarded | |||||
| E5.5* | 8 | 1 abnormal | |||
| 9 | 3 without embryos | ||||
| E3.5† | 23 | 5 | 16 | 2 | |
The C57/129 Fasn+/- males and females were mated, and the day females had vaginal plugs was noted as E0.5. Uteri were removed from the females at various embryonic days after cervical dislocation. The embryos were separated from their yolk sacs and fixed in paraformaldehyde. The yolk sac DNA was used for genotyping by Southern blot analysis. Resorbed embryos could not be genotyped.
The E6.5 and E5.5 embryos were not genotyped, and the litter size for the total embryos refers to decidua. The decidua-containing regions were fixed in paraformaldehyde, and the microscopic observations of the sectioned decidua are described.
Embryos at E3.5 were collected from five mated females and genotyped by PCR followed by Southern blotting analysis with radioactive probes as described in Materials and Methods. The total implanted embryos from E7.5 to E14.5 were: resorbed, 16; +/-, 32; and WT, 26.