Table 1. Mutant alleles of Drosophila fng.
| Wing phenotype of transheterozygotes with
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Allele | DfriXT1 | 80 | 13 | M69 at 18° | 52 | 2 | Mutagen | Mutation | Genetic background |
| 1 | L | L | L | 5.0 (16) | 3.5 (25) | 1.8 (121) | EMS | TCG to ACG S(350) to T | FRT80B P[w+]70C |
| 2 | 2.3 (186) | 1.1 (98) | 2.4 (133) | 2.7 (113) | 1.3 (168) | 1.0 (79) | EMS | CTG to CAG L(398) to Q | st e |
| 3 | L | L | L | 4.9 (14) | 3.2 (58) | 1.9 (122) | EMS | GAA to AAA E(220) to K | FRT80B mwh jv |
| 5 | L | L | L | 5.0 (10) | 4.0 (14) | 2.7 (122) | EMS | TCG to TTG S(350) to* | FRT80B P[w+]70C |
| 7 | L | L | L | ND | 3.9 (29) | 2.4 (159) | EMS | TTG to TAG L(221) to* | st e |
| 8 | L | L | 1 | 5.0 (6) | 3.9 (14) | 2.4 (125) | EMS | AAG to TAG K(196) to* | FRT80B P[w+]70C |
| 11 | L | L | L | ND | 4.2 (16) | 2.6 (84) | EMS | GT to AT K(196) to N E* | FRT80B P[w+]70C |
| 13 | L | L | L | 5.0 (18) | 3.7 (31) | 2.4 (133) | EMS | TGG to TAG W(288) to* | FRT80B mwh jv |
| 14 | L | L | L | ND | 4.3 (8) | 2.7 (134) | EMS | GAT to AAT D(327) to N | FRT80B mwh jv |
| L19 | L | L | L | 5.0 (12) | 3.6 (18) | 2.5 (181) | EMS | TGG to TGA W(181) to* | FRT80B P[w+]70C |
| L73 | L | L | L | 5.0 (30) | 4.1 (23) | 2.8 (127) | EMS | TGG to TAG W(181) to* | FRT80B P[w+]70C |
| L81 | L | L | L | 5.0 (4) | 3.6 (10) | 2.5 (181) | EMS | GGC to GAC G(295) to D | FRT80B P[w+]70C |
| L83 | L | L | L | 5.0 (14) | 3.4 (54) | 2.1 (204) | EMS | CTT to TTT L(213) to F | FRT80B P[w+]70C |
| M69 at 18° | 5.0 (8) | 3.9 (24) | 5.0 (18) | L | 4.2 (29) | 2.7 (113) | EMS | ACG to ATG T(184) to M | FRT80B P[w+]70C |
| M69 at 25° | L | 4.6 (32) | L | L | 2.8 (20) | 2.3 (164) | |||
| M69 at 29° | L | L | L | L | 3.3 (12) | 2.7 (142) | |||
| 35UZ-1 | ND | ND | 0.9 (89) | ND | ND | 0.4 (67) | P element | Insertion | w1118 |
| 52 | 3.3 (34) | 4.6 (10) | 3.7 (31) | 4.2 (29) | 3.0 (41) | 1.3 (168) | Transposase | ND | 35UZ-1 |
| 129 | L | L | L | 5.0 (4) | 3.2 (28) | 2.1 (116) | Transposase | Deletion | 35UZ-1 |
| DfriXT1 | L | L | L | 5.0 (8) | 3.3 (34) | 2.3 (186) | X-ray | Deletion | ru st e ca |
Columns 2-7 show the average phenotypes of combinations of the indicated alleles, with the number of wings scored in parentheses. Phenotypic scores for combinations with fng2 or fng52 that are significantly greater with EMS-induced alleles than with DfriXT1 are indicated in bold, and scores that are significantly less are underlined. L, Lethal; ND, not determined. For point mutations, both the nucleotide changed and amino acid affected are indicated.
stop codon. All EMS-induced mutations except fng2 and fng7 were originally induced on a P[w+] FRT80B chromosome. In addition to these mutations, the two following polymorphisms were identified: in fng2 and fng7, at mRNA nt 690/aa 193, GAG(E) to GAA(E); in fng52 at nt 1605 (3′ UTR), C to A.