Fig. 4.

Exo1 does not induce the ADP-ribosylation of Bars50. The supernatant from a cell lysate of BSC1 cells subjected to high-speed centrifugation was incubated with 10 μM BFA or 100 μM Exo1 in the presence of ³²P-ADP-ribose for 30 min at 37°C. The proteins in the lysate were then subjected to denaturing SDS/PAGE, as demonstrated (11). BFA (lane 2) induces the ADP-ribosylation of Bars50. In contrast, Exo1 (lane 3) had no effect. No ADP-ribosylation is detected in the presence of 1% DMSO (lane 1).