Fig. 3.

Flow cytometric analysis of cryptorchid testis cells stained with Hoechst 33342. (A) Testis SP cells, defined by verapamil sensitivity, are indicated in the box. Shown is Hoechst 33342 staining and emission patterns of whole cryptorchid testis cells in the absence (Left) and presence (Right) of verapamil. (B) Expression of surface markers on the testis SP was analyzed. Testis SP cells express Sca-1 brightly; CD45 and CD34 heterogeneously; and Thy-1, c-kit, and CD43 at negligible levels. Solid line indicates iso-type control antibody stained cells.