The presence of Gβ5-S in the retina and striatum of RGS9–/– mice. Retinal extracts (20 μg) and striatal extracts (CPu, 100 μg) from wild-type and RGS9–/– mice (indicated, Top) were resolved by 12% SDS/PAGE, and the presence of Gβ5 and RGS9 spliced forms were visualized by Western blots by using generic RGS9 antibody (R4432) and Gβ5 antibody (CT-215). Molecular mass markers (top to bottom: 124, 81, 50, and 42 kDa) are shown on the left. The splice variants of RGS9 and Gβ5 are indicated by arrows (top to bottom, RGS9-2, RGS9-1, Gβ5-L, and Gβ5-S).