Fig. 5.

Effector functions of 2C CD8⁺ cells stimulated with Dros APC membrane vesicles. As for proliferation, 2C CD8⁺ cells at 5 × 10⁴ cells per well were stimulated with 100 μg/ml L^d.B7-1.ICAM-1 membrane vesicles loaded with QL9 or p2Ca peptides at the concentrations shown. Concentrations of IFN-γ (a) and IL-2 (b) in culture supernatants collected at 24–60 h are shown; cytokines were measured by ELISA. The data represents means (±SD) of triplicate cultures. (c) CTL activity of 2C cells cultured with vesicles as above for 60 h. The data show mean percent of lysis (±SD) of P815 (L^d) target cells for triplicate cultures; lysis was measured over 4 h. In some cultures, the activated 2C cells were preincubated with 1B2 anti-clonotypic mAb (10 μg/ml). Note that P815 cells were not supplemented with exogenous peptide.