Fig. 1.

Pretreatment with the dual APL increases the size of the CD4⁺CD25⁺ T cells in spleens of SJL mice. SJL mice were injected s.c. with the dual APL (100–500 μg per mouse in PBS) or with PBS three times at 2-day intervals. Splenocytes obtained from these mice were cultured in enriched medium for 44 h and stained with anti-CD4-PE, anti-CD25-FITC Abs, and their matched isotype controls. Results are expressed as the percentage of CD4⁺CD25⁺ T cells, and they represent one experiment of three performed.