Fig. 3.

Depletion of CD4⁺CD25⁺ T cells abrogates the inhibitory effect of the dual APL on the proliferative responses of LN cells of SJL mice. SJL mice were either administered s.c. with the dual APL (200 μg per mouse in PBS) concomitant with immunization with p195–212 (10 μg per mouse in CFA) or immunized with the myasthenogenic peptide alone. Ten days after immunization with p195–212, popliteal LN cells obtained from p195–212-immunized mice (▪) and dual APL-treated mice, before (▵) and after (○) CD25⁺ T cell depletion, were cultured with different concentrations of p195–212, and a proliferation assay was performed as described in Materials and Methods. Results are expressed as mean cpm of triplicates ± SD values, and they represent five experiments performed.