Fig. 7.

The down-regulation of CD28 expression on LN-derived T cells of SJL mice after administration of the dual APL. SJL mice were either administered s.c. with the dual APL (200 μg per mouse in PBS) concomitant with immunization with p195–212 (10 μg per mouse in CFA) or immunized with the myasthenogenic peptide alone. LN-derived T cells were obtained by a procedure of panning and stained with anti-CD28-FITC antibody. Results are presented as percentage of CD28⁺ T cells found in LN 24 h after dual APL administration, and they represent one experiment of three experiments performed.