Fig. 3.
(a) Degree of insulitis was not reduced in class I beta bald mice by histological analysis. Insulitis is scored in the following way: 0, no insulitis; 1, periductal insulitis; 2, circumferential insulitis; 3, intraislet infiltration; and 4, severe structural derangement. The mean scores of female β2Maβ2m+/– HIP-Cre+ (n = 7), β2M–/– (n = 7), class I beta normal (n = 5), and class I beta bald (n = 13) NOD mice are shown. Black bars, 0; diagonal lines, 1; vertical lines, 2; gray, 3; and cross-hatched lines, 4. The proportion of islets exhibiting each level of insulitis severity was not significantly different between class I beta normal and class I beta bald NOD mice (P > 0.05). (b) Content of lymphocytic infiltrate of grade 3 islets from class I beta bald and class I beta normal NOD mice. Consecutive frozen sections taken from 250-day-old female mice were stained with the antibodies 53.6 (CD8 specific), GK1.5 (CD4 specific), and F4/80 (macrophage specific). Sections from β2M+/–, class I beta normal, and class I beta bald NOD mice are shown. (Bar = 10 mm.)