Fig. 1.

Aged ERβ^–/– mice develop myeloproliferative disease. Massive splenomegaly (A) and enlargement of Peyer's patches (B) and mesenteric lymph nodes (C) from 18-month-old ERβ^–/– mice (Left) compared with an age-matched WT littermate (Right) are shown. (D–G) Spleen histology of WT (D) and ERβ^–/– (E) mice and bone marrow histology of WT (F) and ERβ^–/– (G) mice. The femurs were surgically removed and fixed in 4% paraformaldehyde-buffered PBS and decalcified in 5.5% EDTA/10% formalin for 7 days. Nonhematopoietic organs such as liver (H) and lung (I) show a massive infiltration of myeloid cells.