Table 2. Summary of single-cell PCR analysis of bone marrow TLPs.
| TCRβ
|
||||
|---|---|---|---|---|
| Germ line | D-J | V-DJ | No product | |
| CD7+ CD10+ TLPs | ||||
| Donor I | 12/40 | 9/40 | 9/40 | 10/40 |
| Donor II | 4/12 | 2/12 | 4/12 | 2/12 |
| Donor III | 5/12 | 2/12 | 2/12 | 3/12 |
| Donor IV | 7/12 | 1/12 | 2/12 | 2/12 |
| Donor V | 1/12 | 5/12 | 4/12 | 2/12 |
| Total | 29/88 | 19/88 | 21/88 | 19/88 |
| TCRαβ+ T cells* | ||||
| With donor I | ND | ND | 10/12 | 2/12 |
| With donors II-V | 0/12 | 1/12 | 11/12 | 1/12 |
| Total | 0/12 | 1/12 | 21/24 | 3/24 |
| Buffer controls* | ||||
| With donor I | 0/8 | 0/8 | 0/8 | 8/8 |
| With donors II-V | 0/12 | 0/12 | 0/12 | 12/12 |
| Total | 0/20 | 0/20 | 0/20 | 20/20 |
ND, not done.
*
Amplified in parallel as indicated.