Fig. 2.

CD4 T helper responses in HCV-LP- or DNA-immunized AAD mice. AAD mice were immunized, and splenocytes were harvested 2 wk after the last immunization as described in Materials and Methods.(A) Proliferative response. Splenocytes were stimulated in vitro for 5 days with 5 μg/ml recombinant HCV core or E1/E2 proteins. Data from individual mice are shown, and the mean SI ± SEM is indicated as bar on the left. SI of >3 (dotted line) is considered positive. IFN-γ (B) and IL-4 (C) ELISpot assays of splenocytes after stimulation with recombinant HCV core or E1/E2 protein are shown. Spots from individual mice are shown, and the mean ± SEM is indicated as bar on the left. The P values are shown. These data are representative of three similar experiments.