Fig. 6.

Vaccinia titer in adoptive transferred BALB/c mice. Ten BALB/c mice were immunized three times with HCV-LP. Two weeks after the third immunization, splenic lymphocytes were harvested and pooled. After washing with PBS extensively, CD4 or CD8 cells were depleted. Cells (3 × 10⁷) with or without depletion were injected into syngenic naive BALB/c mice through the tail vein. After adoptive transfer (24 h), the mice were challenged with 10⁶ pfu of vvHCV.S i.p. Mice were killed 4 days after challenge, and then vaccinia titers in ovaries were determined. Data are shown for each mouse, and the mean vaccinia titer ± SEM is indicated. The P values are shown. These data are representative of three similar experiments.