Fig. 2.

LCM of neurons and astrocytes from hippocampus of mouse brain. A Nissl-stained section from the brain of a normal mouse (A), the section after laser microdissection and capture of neurons (B), the isolated neurons on the capture membrane (C), and the neurons overlying the dissected section (D) are shown. A brain section in which fluorescein-conjugated anti-GFAP has been used to identify astrocytes with fluorescent microscopy is shown in E, and the same tissue after laser capture of the stained cells is shown by light microscopy in F; the captured cells under light are shown in G, and fluorescence is shown in H.