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. 2003 May 16;100(11):6795–6800. doi: 10.1073/pnas.0936797100

Fig. 2.

Fig. 2.

Effect of adrenaline on mPer1 gene expression in liver slices in vitro. mPer1 mRNA was expressed in a manner dependent on the concentration of adrenaline (A), phenylephrine (B), and isoproterenol (C). The vertical values reflect the relative mRNA levels of mPer1 or mPer2 as the ratio to β-actin mRNA levels. mPer1 or mPer2 levels in the vehicle (V)-treated group are set at 1.0. Each point represents three to six animals. *, P < 0.05; Dunnett's test. (D and E) Effect of mitogen-associated protein kinase (MAPK) kinase (D) and protein kinase A (PKA) (E) inhibitor on adrenaline-induced mPer1 gene expression. Each point represents three to six animals. *, P < 0.05; Student's t test. (F) In vitro study of the effect of adrenaline (0.1 μg/ml, for 60 min) on RLU and mPer1 mRNA expression in the liver of transgenic mice carrying the mPer1 promoter-luciferase. Both bioluminescence (RLU) and RT-PCR products of the intrinsic mPer1 gene transcript were measured in hepatic slices (12 slices from three animals for each point). Images of mPer1 and β-actin RT-PCR products after electrophoresis are shown above each figure. **, P < 0.01, Student's t test.