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Modified sequence element and assembled nucleosome structure. (A) GRE sequence for UO and UI substrates. Bold arrows are the pseudo-palindrome binding site for the glucocorticoid receptor. (B Top) Nucleosome schematic of the TG motif (gray) flanking the GRE (maroon and gold). (Middle and Bottom) UO and UI (red) substrates (B was adapted from ref. 4). The rotational setting of DNA in these NuCPs has been determined by hydroxyl radical footprinting (10).
