Fig. 2.
Nucleosome reconstitutions. Percent of NuCPs present for different CE-CP to [32P] DNA ratios. Data represent the mean ± 1 SD (three independent reconstitutions for each substrate) of the ratio of band intensities for naked DNA and NuCPs on native gels (Inset). Undamaged DNA, solid line, ○; UO DNA, dashed line, ▪, and UI DNA, dotted line, ⋄. (Inset) Native gels of nucleosome reconstitutions at varying CE-CP concentration (naked DNA, dashed arrow; NuCP, solid arrow). Lane M is naked DNA only; the black triangle indicates increasing CE-CP concentration.
