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. 2003 Jun 10;100(13):7465–7470. doi: 10.1073/pnas.1330328100

Fig. 3.

Fig. 3.

UDG and APE digestion of UO and UI nucleosomes. (A) Denaturing gels of UO and UI naked DNA and NuCPs after incubation with UDG and APE, each at 1 nM. Time is from 30 sec to 1 h and M is mock-treated. (B) Plot of the mean ± 1 SD (three independent experiments) of the percent DNA cut by 1 nM UDG and APE for UO DNA (solid line, •), UI DNA (dashed line, □), UO NuCP (solid line, ⋄), and UI NuCP (dashed line, ▵). Dotted line (×) represents nucleosome samples digested with 10 nM UDG and APE. (C) Plot of the mean ± 1 SD (three independent experiments) of the percent of DNA cut by 100 pM UDG and APE for UO DNA (solid line, □) and UI DNA (dashed line, □). (Inset) Representative gel for digestions of UO and UI naked DNA for times from 30 sec to 10 min.