. 2003 Jun 9;100(13):7471–7475. doi: 10.1073/pnas.1332771100

Table 1. Binding and aminoacylation parameters of microhelix^Ala with 461N and fusion proteins 368N-MFβ21 and 368N-MFβ25.

Construct	K_d, μM	K_m, μM	k_cat, min^-1	k_cat/K_m, 10^-3	Loss in efficiency
461N (C-terminal 6-His)	3.100	100	100	1,000	1
Fusion proteins
368N-MFβ21	0.075	150	0.25	1.66	1/600
368N-MFβ25	1.500	150	0.1	0.66	1/1,500

Kinetic parameters were determined at pH 7.5, 37°C. K_d values were determined at 4°C.

Table 1. Binding and aminoacylation parameters of microhelixAla with 461N and fusion proteins 368N-MFβ21 and 368N-MFβ25.