Fig. 2.

Dependence on hexose concentrations of inhibition by compound 3361. (a Upper) Effect of compound 3361 on [³H]hypoxanthine incorporation in cultured parasites at different d-glucose (Left) and d-fructose (Right) concentrations. Red, blue, pink, orange, and green lines correspond respectively to 4, 6, 8, 10, and 12 mM glucose and 20, 25, 30, 35, and 40 mM fructose in culture medium. (Lower) Change in IC₅₀ for compound 3361 depending on the d-glucose (Left) and d-fructose (Right) concentrations. Data from three independent assays are shown (for glucose r² = 0.84 and P < 0.0001; for fructose r² = 0.80 and P = 0.0002). Values obtained with the multidrug-resistant strain K1 at extremes of d-glucose concentrations are represented by asterisks. (b) Effect of compound 3361 on the morphology of P. falciparum in culture (30μM compound 3361; 4 mM extracellular glucose) and of P. berghei in mice. Arrows show vacuole formation.