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. 2003 Jun 13;100(13):7491–7496. doi: 10.1073/pnas.0932835100

Fig. 3.

Fig. 3.

Subcellular localization of PTEN and its mutants in HEK293T and HeLa cells. PTEN and its mutants tagged with EGFP at their C termini were transiently transfected into HEK293T (AG) and HeLa (HJ) cells, and their subcellular localization was monitored by confocal microscopy. (A) C2 domain. (B) Δ353–403/C124A. (C) Δ1–185. (D) Δ353–403/C124A/R161A/K163A/R164A. (E) Phosphatase domain (C124A). (F) C124A–PTEN. (G) C124A/S380E/T382E/T383E. (H) C124A/S380A/T382A/T382A. (I) C124A/Δ400–403. (J) C124A/S380A/T382A/T383A/Δ400–403. (K) C124A (HeLa). (L) C124A/S380A/T382A/T382A (HeLa). (M) Δ400–403/C124A/S380A/T382A/T383A (HeLa).