Dynamics of the cruciform observed with time-lapse AFM for hydrated sample t3low (frames a-k) and sample t7high (frames l-v) in HE buffer.
Initial image of the molecule with cruciform is shown in frame a (sample t3low) and frame l (sample t7high). Arrow is pointed to the cruciform. Images of enlarged area specified by the square in initial image, show time progress of the cruciform structure (frames b-f for sample t3low and frames m-q for sample t7high). Traces of the same molecule on black background are shown in frames g-k (sample t3low) and frames r-v (sample t7high). The capture time for images are 0 min (a), 11 min (b), 44 min (c), 60 min (d), 134 min (e), 155 min (f) (sample t3low) and 0 min (l), 13 min (m), 22 min (n), 71 min (o), 233 min (p), 363 min (q) (sample t7high).