Fig. 5.

Effect of C179S mutation on the fibril formation of the PrP175–195 fragment. (Left) Aggregation kinetics for C179S in the presence of 10 mM DTT (red) and absence of DTT (blue), and for wild-type PrPUP incubated with 10 mM DTT (dashed line with filled squares) and absence of DTT (dashed lines with open squares). (Right) UV-visible spectra of Congo red (dotted line) and Congo red titrated with C179S (solid line) and PrPUP (dashed line) after 24 h of incubation in the absence of DTT. (Inset) Visual inspection of Congo red solutions titriated with the mutant (S) and wild-type (WT) solutions after 24 h of incubation in the absence of DTT.