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. Author manuscript; available in PMC: 2006 Nov 17.
Published in final edited form as: Nat Neurosci. 2003 Jul;6(7):708–716. doi: 10.1038/nn1074

Figure 7.

Figure 7

Clustering of neurexin is sufficient to induce recruitment of synaptic vesicles. (a–i) Pre-clustered anti-VSV multimers were added to 12-day-old hippocampal cultures transfected with VSV–neurexin (a–c), VSV–neurexin-delta-C (d–f) and VSV–L1 (g–i). Antibody clustered proteins (a, d and g, green in the overlay) and the distribution of synapsin (b, e and h, red in overlay) are shown. The integrity of all cells and the cellular processes was controlled by DIC microscopy to ensure that the cells remained intact during the procedure. Scale bar, 10μm. (j) Quantitation of synapsin accumulation in response to clustering of membrane proteins with antibodies. The percentage of recognizable antibody-induced clusters that showed accumulation of synapsin was determined (n ≥ 140 puncta from ≥ 4 cells for each construct). The mean ± s.d. for cells expressing VSV–neurexin (NRX), VSV–neurexin lacking the cytoplasmic tail (ΔC), VSV–N-cadherin (N-cad) and VSV–L1CAM (L1) are shown. See Supplementary Fig. 5 online for additional data.