Expression of mRNA in cross sections of rat cochlea at postnatal day 10 (A), mouse cochlea at postnatal day 7 (B), and the mosquito T. brevipalpis (C and D) using in situ hybridization with rat prestin-specific riboprobes. Note the restricted localization of prestin mRNA to the outer hair cells (OHC) in rat and mouse cochlea (three arrows in row). IHC, inner hair cell. (C) In cross sections of T. brevipalpis antennae, hybridization signals occur in the second antennal segment (pedicel). (D) Higher magnification of positive signals localizes hybridization to chordotonal sensilla, the auditory mechanosensory units of Johnston's organ (JO). (E and F) No signals were observed with the sense probe.