Sequence alignment of the predicted amino acid sequence of the Drosophila CG5485 and the D. rerio Zebrafish_I gene product with rat prestin. Identical amino acids are shown in white type on a black background, strongly similar amino acids are shown in white type on a gray background, and weakly similar amino acids are shown in black type on a gray background. Rat prestin and Drosophila_CG5485 share an identity of 26.5%, a strong similarity of 49.4%, and a weak similarity of 61.4%. Rat prestin and Zebrafish_I share an identity of 49.8%, a strong similarity of 68.4%, and a weak similarity of 76.6%. The conserved sulfate anion transport motif is boxed (compare with Fig. 2B); the region with highest homology within the rat prestin riboprobe is dot-boxed (compare with Fig. 2 A).