Fig. 6.

TJ expression by jejunal epithelial cells from naïve or d9 post-T. spiralis-infected mice. Shown are TJ proteins in epithelium of intestinal villi visualized by confocal microscopy. (a) Dual staining of occludin (O) and desmoplakin (Dp) shows that both localize to the plasma membrane of naïve enterocytes. (b) Occludin (green) is internalized by enterocytes from d9 postinfected (p.i.) mice whereas desmoplakin (red) remains within the plasma membrane. (cA) Representative Western blot of occludin expression in epithelial tissue from naïve mice compared with d9 postinfection mice. (cB) A Coomassie blue-stained gel shows equal loading of total protein. (cC) Four Western blots of occludin from naïve or infected mice were analyzed by densitometry and the mean density of the 66-kDa band ± SEM is shown. *, P < 0.05. (d–g) Jejunal villi from naïve or infected mice were stained for ZO-1 (d and e) or claudin-1 (f and g).