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. 2003 Jun 16;100(13):7797–7802. doi: 10.1073/pnas.1330920100

Fig. 1.

Fig. 1.

Transfection of siRNA targeted against caspase 8 mRNA prevents FasL-mediated apoptosis of HepG2 hepatoma cells. HepG2 cells were transfected with either a nonsense siRNA or a specific siRNA targeted against caspase 8. After 48 h cells were infected with AdFasL or AdGFP. (a) Caspase 8 assay (caspase 8 sequence 1). Cells pretreated with caspase 8 siRNA show ≈2.5-fold reduced caspase 8 activity compared with cells pretreated with scrambled siRNA 8 h after adenoviral infection. (b) Histone ELISA (caspase 8 sequence 1). Caspase 8 siRNA treatment inhibits Fas (CD95)-mediated apoptosis as shown by photometric histone ELISA 12 h after infection. The figure shows representative data obtained from three independent experiments.