Table 1. Phagocytosis of L monocytogenes, S. cerevisiae, and opsonized and nonopsonized thymocytes by WT and TGase2^-/- macrophages (MΦ).

	WT MΦ with ingested particles, %	TGase2-/- MΦ with ingested particles, %
WT apoptotic thymocytes	76.7±8.2	51.1±4.7*
TGase2-/- apoptotic thymocytes	77.1±9.1	51.7±3.7*
Nonopsonized thymocytes	7.1±1.0	6.2±0.8
Thymocytes opsonized with anti-CD3 mAb	46.7±9.8	49.1±9.9
Thymocytes with isotype control mAb	14.1±2.3	11.7±1.6
L. monocytogenes +50 μM	77.6±12.1	82.7±13.1
cytochalasin B	17.6±5.2	14.7±6.9
S. cerevisiae +50 μM	67.0±20.4	68.9±8.4
cytochalasin B	22.8±11.7	24.7±14.1

Each experimental group included three independent experiments with macrophages isolated from separate mice. In the case of opsonized thymocytes, cells treated with isotype control mAb served as control for nonspecific binding, whereas in the case of bacterial and yeast phagocytosis, treatment with cytochalasin B, an inhibitor of phagocytosis, was used for discrimination of nonspecific interaction with macrophages. Data represent mean ± SD

^*Significantly different from the WT (P < 0.002)