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. 2003 Jun 16;100(13):7818–7823. doi: 10.1073/pnas.1337066100

Fig. 3.

Fig. 3.

Expression and methylation status of CHFR in DNMT knockout cells. (A) Re-expression of CHFR in methyltransferase knockout cells. The genotypes of the cell lines are indicated: MT1KO1 and MT1KO2, DNMT1-/-; 3bKO1 and 3bKO2, DNMT3b-/-; DKO2 and DKO3, DNMT1-/-DNMT3b-/-. (B) Bisulfite PCR analysis of the methylation status of the CHFR CpG island in HCT116 and methyltransferase knockout cells; M, methylated alleles. (C) Bisulfite sequencing of CHFR in WT and DNMT knockout cells. The PCR products were cloned into a vector, and at least nine clones were sequenced for each cell line. Open and closed areas represent unmethylated and methylated CpG dinucleotides, respectively.