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. 2003 Jun 9;100(13):7871–7876. doi: 10.1073/pnas.1230643100

Fig. 2.

Fig. 2.

Characterization of the low-mobility form of the d109 genome. (A) d109 virions (lane 1), d109-infected cells (lane 2), and Raji cells (lane 3) were analyzed on a Gardella gel and probed for HSV and EBV genomes. (B) Template for eluting regions a, b, c, and d from a Gardella gel of d109 virions (lane 4) and d109-infected cells (lane 5). (C) The DNA in samples a–d in B were analyzed as described in Materials and Methods to observe the abundance of joint (J) and terminal (T) fragments. Different amounts of d were loaded on the gel. (D) Vero cells were infected at the indicated moi of d109 for 24 h, run on a Gardella gel, subjected to Southern blot analysis, and quantified. (E) The kinetics of circularization of d109 genomes in Vero cells was determined as described in the text.