Table 1. Crystallographic data.
| Crystal | λ, Å | Resolution, Å | I/σI* | Rm,† % | Multiplicity‡ | Completeness, %§ | PhP¶ | |
|---|---|---|---|---|---|---|---|---|
| P. aeruginosa SulA, space group P212121, a = 79.7 Å, b = 91.7 Å, c = 92.3 Å | ||||||||
| OCMP | 0.8638 | 3.5 | 12.9 (4.4) | 0.095 (0.274) | 3.9 | 99.8 (99.8) | 1.3 | 1.4 |
| K2OsO4 | 0.8638 | 4.0 | 13.3 (4.4) | 0.103 (0.217) | 3.8 | 94.8 (94.8) | 0.7 | 0.5 |
| KAu(CN)2 | 1.4880 | 3.5 | 12.7 (3.5) | 0.076 (0.335) | 4.0 | 99.9 (100.0) | 0.5 | 0.2 |
| NATI | 0.9330 | 2.9 | 14.0 (3.0) | 0.075 (0.336) | 3.1 | 95.9 (95.9) | ||
| P. aeruginosa SulA:P. aeruginosa FtsZ, space group C2, a = 78.0 Å, b = 54.0 Å, c = 250.7 Å, β = 101.1° | ||||||||
| PEAK | 0.9793 | 3.9 | 18.2 (6.6) | 0.064 (0.194) | 10.8 (5.4) | 96.4 (96.0) | ||
| INFL | 0.9797 | 3.9 | 17.2 (5.9) | 0.065 (0.216) | 10.4 (5.2) | 96.5 (96.5) | ||
| HREM | 0.9393 | 3.9 | 18.7 (9.0) | 0.068 (0.131) | 10.6 (5.3) | 96.4 (96.4) | ||
| P. aeruginosa SulAΔ35:P. aeruginosa FtsZ, space group P212121, a = 55.6 Å, b = 75.4 Å, c = 241.0 Å | ||||||||
| NATI | 0.9393 | 2.1 | 11.4 (3.7) | 0.096 (0.334) | 3.8 | 99.7 (99.7) | ||
Signal-to-noise ratio of merged intensities, in brackets for the highest-resolution shell
Rm: ΣhΣi|I(h, i) - I(h)|/ΣhΣiI (h, i), where I(h, i) are symmetry-related intensities, and I(h) is the mean intensity of the reflection with unique index h, in parentheses for the highest-resolution bin
Multiplicity for unique reflections, anomalous multiplicity in brackets
Completeness for unique reflections; anomalous completeness is identical, because inverse beam geometry was used, in parentheses for the highest-resolution bin. Correlation coefficients of anomalous differences at different wavelengths for the MAD experiment: PEAK vs. INFL, 0.47; PEAK vs. HREM, 0.57; INFL vs. HREM, 0.31
PhP, phasing power; acentric reflections for isomorphous and anomalous phasing, respectively. Figure of merit for all acentric reflections is 0.39