Fig. 4.

4a attenuated IL-1β-mediated, but not LPS-mediated, effects in freshly isolated lymphocytes. Freshly isolated murine lymphocytes were treated similarly as EL4 cells with either IL-1β (A and B) or LPS (10 ng/ml, 30 min; C and D). MAP kinase activation (A and C) and the association of MyD88 with either IL-1RI (B) or TLR4 (D) were analyzed as described in Fig. 1. n = 3–8 independent experiments. The * indicates statistical significance with respect to control, and the # indicates statistical significance with respect to IL-1β at P < 0.05 by ANOVA followed by Tukey test.