TABLE 1.
Plasmid (pBD) | Background | Mutation(s)a |
---|---|---|
1437 | pRS316 | S160D |
2659 | pRS316 | T169D, S170D, S547D |
2663 | pRS316 | T169A, S170A, S547A |
2664 | pRS316 | S149A, S152A, T169A, S170A, T267A, S268A, S547A |
2667 | pRS316 | L549A |
2668 | pRS316 | I545A |
2669 | pRS316 | T169A, S170A, T267A, S268A, S547A, S715A, T743A |
2673 | pZUC12 | S547A |
2742 | pZUC12 | T169D, S170D, S547D |
2675 | pGEX2T-1 | T267A, S268A |
2677 | pGEX2T-1 | I545A |
2676 | pGEX2T-1 | L549A |
2678 | pGEX2T-1 | S547A |
2681 | pGEX2T-1 | S547D |
2682 | pGEX2T-1 | T556A, T557A |
2680 | pGEX2T-1 | T169D, S170D |
2686 | pGEX3X-1 | S715E |
2687 | pGEX3X-1 | T743A |
Mutation designations consist of the wild-type amino acid, its position, and then the substituted amino acid.