TABLE 1.
Target mRNA | Oligonucleotide(s) |
---|---|
Env-Wa | (+) CGGACATCCAAAGTGATACATCCT, (−) TGATGTATCCAAGACTCCACTCCA |
RPLP0a | (+) GGCGACCTGGAAGTCCAACT, (−) CCATCAGCACCACAGCCTTC |
hCGβa | (+) GCTACTGCCCCACCATGACC, (−) ATGGACTCGAAGCGCACATC |
Env-W 744-766b | GCCATGGGGATTTATGATTTTAG |
Env-W 750-770b | GAGGGCCATGGGGATTTATGA |
Env-W 758-777b | GATAAGGGAGGGCCATGGGG |
Scrambledb | GNNNNNNNNNNNNNNNNNNG |
Upper (+) and lower (−) primers used in RT-PCR assays.
Phosphorothio (underlined) antisense oligonucleotides used to modulate gene expression. The Env CAT initiation anticodon is indicated in boldface; N, fully degenerated base.