FIG. 7.

KIX disrupts the recruitment of p300 HAT activity to chromatin templates in a Tax-dependent manner. Both chromatin-assembled templates and free histones were acetylated in vitro in the presence of p300 and [¹⁴C]acetyl-CoA. Tax/CREB, GST-KIX, and GST-KIXmut were added to the indicated acetylation reaction mixtures. The CBP/p300-specific HAT inhibitor Lys-CoA was added to a final concentration of 50 μM, as indicated. GST-KIX and GST-KIXmut were each added at a fivefold molar excess relative to the Tax/CREB complex.