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. 2003 May;23(10):3392–3404. doi: 10.1128/MCB.23.10.3392-3404.2003

FIG. 8.

FIG. 8.

KIX, SREBP-1a, and Lys-CoA inhibit Tax-activated transcription on chromatin templates lacking histone amino-terminal tails. Transcription reaction mixtures contained the p4TxRE-G-less template assembled into chromatin by using tailless histones, CEM nuclear extract, and Tax/CREB, as indicated. The recovery standard and full-length G-less transcripts are indicated. (A) The KIX polypeptide specifically inhibits Tax/CREB transcription on tailless chromatin. GST-KIX or GST-KIXmut was added at an equimolar concentration (+) or fivefold molar excess (++) relative to the Tax/CREB complex, as indicated. (B) SREBP-1a specifically inhibits Tax/CREB transcription on tailless chromatin. GST-SREBP-1a and GST were added at equimolar concentration (+) or fivefold molar excess (++) relative to the Tax/CREB complex, as indicated. (C) Exogenous p300 relieves KIX and SREBP-1a-mediated inhibition of Tax/CREB transcription on tailless chromatin. GST-KIX and GST-SREBP-1a were added to the indicated transcription reaction mixtures at fivefold molar excess (++) relative to the Tax/CREB complex. Recombinant p300 was added at 0.05 and 0.1 pmol. (D) Lys-CoA inhibits Tax/CREB transcription on tailless chromatin. For this experiment, acetyl-CoA was added to a final concentration of 10 μM in all samples. Lys-CoA was added to samples at final concentrations of 10 μM (+) and 50 μM (++).