FIG. 5.

Confirmation of cDNA microarray results. Total RNA was prepared using Trizol reagent (Life Technologies), according to the instructions given by the manufacturer, from wild-type and DN2-TG mice treated with vehicle alone or 50 mg of T0901317/kg for 6 days (A), as well as Hepa-1c1c7 (B) and RAW264.7 (C) cells treated with either vehicle alone or 10 μM T0901317 for 16 h, as indicated. Semiquantitative RT-PCR was used to determine the relative levels of mRNA for insig-2, PPARγ, IKKɛ, IKKα, IKKβ, and GAPDH. #4, DN2-TG mouse line no. 4.