FIG. 6.

Serpin gene expression in Fao-1 hybrids containing various mutant serpin alleles. A serial twofold dilution series of cytoplasmic RNA from F(14n)14, a rat hepatoma hybrid containing a wild-type human chromosome 14, is shown in the lanes on the left. RNAs from parental Fao-1 cells and from three to four independent hybrid clones containing the Δ3.4, Δ2.0, Δ8.0, Δ4.3, and Δ2.3 mutant chromosomes are also shown. The number above each column identifies the clone within a series. Human α1AT and CBG expression was down-regulated in the Δ8.0, Δ4.3, and Δ2.3 series hybrids, but rat α1AT was expressed in all the hybrid clones. Rat cyclophilin served as an RNA loading control.