Chromatin organization of the Δ3.4, Δ4.3, Δ2.0, and Δ2.3 mutant alleles. Expression-associated DHSs that were present are indicated by gray arrows, constitutive DHSs are indicated by black arrows, DHSs that were deleted are indicated by open arrows, and DHSs that failed to form are indicated by X's. The Southern blots used to assay specific DHSs are shown linked to the relevant site(s) on the map. The Δ3.4 and Δ2.0 mutant alleles had wild-type patterns of DHSs throughout the region, but the Δ4.3 and Δ2.3 mutant alleles each affected formation of a distinctive set of DHSs.