FIG. 4.

fNMHCB has cytoplasmic retention properties. HEK293T cells were transfected with GAL-DBD or GAL-fNM and double labeled with anti-GAL-DBD (a to h) and anti-PBX1 (a′ to h′) antibodies. The two sets of photomicrographs show the same cell(s) visualized for GAL and PBX1 immunoreactivities (e.g., a and a′). The bar graphs display the percentages of cells showing nuclear (N), nuclear and cytoplasmic (NC), and cytoplasmic (C) localization of GAL species (f, g, h) and PBX1 (f′, g′, h′). The values are the averages of three independent experiments; 150 to 200 cells from each transfection were scored for the localization of the protein of interest. The error bars show standard deviations.